In our hands, the genelute miniprep kits yield sufficient amounts of highly pure plasmid dna. Polyethylenimine based magnetic nanoparticles mediated non. Transformants were scraped into luria broth plus carbenicillin, grown overnight and then dna was extracted using the genelute hp plasmid maxiprep kit sigma. The kit features a filter syringe for the rapid clearing of lysate and a silica binding column designed for either a vacuum or a spin format.
The quality of isolated dna has been evaluated in a set of tests. Endofree plasmid maxi kit from qiagen was used to isolate 7. Genelute plasmid miniprep kit none no the hazards identified with this kit are those associated with the following substances. The genelute hp 96 well plasmid miniprep kit offers a. This is just a small selection of products available from sigmaaldrich. Sigma aldrich products are sold exclusively through sigma aldrich, inc. The genelute hp plasmid maxiprep kit offers a simple, rapid, and cost effective method for isolating plasmid dna from recombinant e.
Genelute plasmid miniprep kit none no the hazards identified with this kit are those associated with. Thermo scientific genejet plasmid maxiprep kit, sufficient for 25 preps, utilizes silicabased membrane technology in the form of convenient spin column. Genelute hp plasmid maxiprep kit from sigmaaldrich. The genelute hp plasmid midiprep and maxiprep kits offer purification of highquality plasmid dna in less than 30 minutes. Production and purification of hightiter newcastle. What is your preferred maxi prep kit for plasmid dna grown in. Please consult the material safety data sheet msds for information regarding hazards and safe handling. Na08001kt genelute hp endotoxinfree plasmid gigaprep kit 418.
Genelute plasmid miniprep kit 2 3 4 prepare cleared lysate add 350 l of neutralization solution s3. Thermo scientific genejet plasmid maxiprep kit for 25. Certificate of analysis thermo scientific genejet endofree plasmid maxiprep kit is qualified by isolating high copy number plasmid dna from 350 ml of overnight e. Plasmid midiprep kit and sigma genelute hp plasmid. The pureyield plasmid maxiprep system is designed to isolate highquality plasmid dna for use in eukaryotic transfection and in cellfree expression experiments. Buy and find information on the genelute hp plasmid dna maxiprep kit with 25 purifications great for plasmid purification at. Here we compare the pureyield miniprep, midiprep and maxiprep systems to other plasmid purification systems. The genelute plasmid miniprep kit offers a simple, rapid, and costeffective. Click on the product or protocol links to be directed to the manufacturers pages. Genelute hp plasmid maxiprep kit from milliporesigma, a. The luciferase assay system was purchased from promega madison, wi, usa.
Analysis of combinatorial cis regulation in synthetic and. A technical bulletin on genelute hp high performance plasmid midiprep kit na0200, from sigmaaldrich. Plasmid maxiprep kit offers a patent pending technology that allows for the rapid. Upon addition to reaction mixtures, in the presence of. High efficiency method to obtain supercoiled dna with a commercial. To reorder product call 18003253010, visit our web site at, or contact your. Each prep recovers up to 750 g of high copy plasmid dna that can be used in a wide variety of molecular biology procedures, such as restriction en.
For additional information, please refer to the individual material safety data sheets. Production and purification of hightiter newcastle disease. For large plasmids, like the ndv genome plasmid, grow bacterial cultures at 30c in the presence of carbenicillin rather than ampicillin, and culture bacteria for 24 hr rather than the usual 16 hr. Ldopa and da were dissolved in double distilled water as 12 mm stock solutions prior to experimentation. Genelute hp endotoxinfree plasmid maxiprep kit sigmaaldrich. Plasmid dna maxiprep kit plasmid dna maxiprep kit norgens plasmid dna maxiprep kit is designed for the rapid preparation of plasmid dna from up to 100 ml of escherichia coli culture. Converting pcdnadcas9p300 core for use with gateway recombination technology addgene plasmid 657 was prepared as above and digested with asci and pmei restriction. Sigmaaldrich products are sold exclusively through sigmaaldrich, inc. The kit allows for the isolation of plasmid dna with final endotoxin levels of 0. The endofree maxiprep kit from qiagen or the genejet maxiprep kit from thermo fisher were used for maxi preparations. Midiprep kit prepsna0200 genelute hp plasmid midiprep kit 25 preps na0300s genelute hp plasmid maxiprep kit prepsna0300 genelute hp plasmid maxiprep kit 10. Qiagen qiagen plasmid maxi kit 12162, 12163, 12165, hispeed plasmid maxi kit 12662, qiagen plasmid plus maxi kit 12963, 12965, endofree plasmid maxi kit 12362, qiafilter plasmid maxi kit 12262, 12263, compactprep plasmid maxi kit 12863 sigma genelute hp plasmid maxiprep kit na0300, na0310. The genelute hp plasmid midiprep kit offers a alkalinesds lysis.
The kit uses a vacuum format with a filter column for the rapid clearing of the bacterial lysate and a silica column for capturing plasmid dna. Trueblue lysis buffer an optional color indicator is included with the kit in order to prevent common handling errors, ensuring. The kit is designed to reduce endotoxin level to na0200 bulletin free download as pdf file. Characterization and evaluation of the efficacy of. Trueblue lysis buffer an optional color indicator is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. We wound up trying several different plasmid prep kits, and the clear winner in our hands was the sigma genelute hp plasmid maxiprep kit.
Genelute hp endotoxinfree plasmid maxiprep kit vs zymopure. Most commonly ordered new lab items weve put together this quick checklist to assist you in setting up your new lab. Thermo scientific genejet plasmid miniprep kit has been tested in the isolation of puc19 dna from e. Thermo scientific genejet plasmid maxiprep kit is qualified by isolating high copy number plasmid dna from 250 ml of overnight e. The concentration of plasmid dna pdna solution was determined by using the nanodrop method. The purpose of plasmid purification was to overexpress insert protein in. Genelute hp endotoxinfree plasmid maxiprep kit from sigma, fastest, easiest and cheapest with great quality. Sigmaaldrich offers a number of genelute hp plasmid maxiprep kit products. Plasmid dna maxiprep purification kits and reagents biocompare. Plasmid dna maxiprep kit product insert norgen biotek. The protocol below is meant to describe the general procedure for purifying plasmid dna from bacterial cultures. The maxiprep kit protocol will typically yield 500850 g plasmid dna from 100200 ml bacterial culture, at a purity that is comparable to that achieved by two passes through cesium chloride gradients. Plasmid dna extraction maxiprep kit 3 bio basic plasmid dna extraction maxiprep kit is an excellent tool offering a rapid and economic method to purify plasmid dna from bacteria cultures.
This system applies salt free reagent to get high pure plasimd, no salt remain, and the concentration of endotoxin below 0. Endofree plasmid maxiprep kit the tonkbio endofree plasmid maxi kit is designed for efficient and convenient extraction of endofree dna. Minipreparation of plasmid dna is a rapid, smallscale isolation of plasmid dna from bacteria. The genejet endofree plasmid maxiprep kit is designed for large scale isolation of high quality endotoxinfree plasmid dna from recombinant e. Plasmid dna maxiprep purification kits and reagents. All materials were supplied by sigmaaldrich corporation st. Plasmids were prepared by using genelute hp plasmid maxiprep kit. The readytouse plasmid dna is of high quality in lowsalt tris buffer and suitable for typical downstream applications. Tcefs pi university of massachusetts medical school. In increasing order, these are the miniprep, midiprep, maxiprep, megaprep, and gigaprep. We found that the genelute hp plasmid maxiprep kit sigma was the only kit that did not shear the ndv genome plasmid. What is your preferred maxi prep kit for plasmid dna grown in bacteria. The purelink hipure plasmid maxiprep kit is designed to isolate transfectiongrade plasmid dna from e. Xtremegene hp dna transfection reagent highperformance polymer reagent for transfecting many cell lines.
Wootton 1department of pathobiology, university of guelph, guelph, on n1g 2w1, canada. The plasmid miniprep kit is based on the alkaline lysis of bacterial cells, followed by the binding of the dna onto the glass fiber matrix of the spin column in the presence of high salt. In most cases the pureyield systems outperform several other systems in terms of speed of purification, as well as quality and downstream performance of plasmid dna. Product information thermo scientific genejet plasmid. Nov 23, 2008 transformants were scraped into luria broth plus carbenicillin, grown overnight and then dna was extracted using the genelute hp plasmid maxiprep kit sigma. Qiagen qiagen plasmid maxi kit 12162, 12163, 12165, hispeed. Characterization and evaluation of the efficacy of cationic. Compare plasmid dna maxiprep purification kits and reagents from leading suppliers on biocompare. Mirapreps and minipreps were conducted using either genejet from thermo fisher waltham, ma, usa, qiagen hilden, germany, or genelute from sigma st. Genelute plasmid prep kit from sigmaaldrich biocompare. Millipore sigma purification kits crossreference tool from. The genelute plasmid miniprep kit offers a simple, rapid, and costeffective method for isolating plasmid dna from recombinant e. The microbca protein assay kit was purchased from pierce rockford, il, usa. Xtremegene hp dna transfection reagent sigmaaldrich.
Genelute hp high performance plasmid midiprep kit na0200. The plasmid miniprep kit provides a fast, simple, and costeffective method for the plasmid dna isolation from the cultured bacterial cells. The genelute hp endotoxinfree plasmid maxiprep kit was obtained from sigmaaldrich. Genelute hp plasmid kits include a filter syringe for lysate clearing, binding columns that can be used with a vacuum or spin purification format, and does not. If you want to perform plasmid purification without using a kit, you can find a protocol for kitfree plasmid miniprep at the bottom of this page. Each of the components can be purchased individually which means you can again make the kit work for you. The plasmid dna yield will vary depending on the plasmid copy number, type and size, the bacterial strain, the growth conditions, and the kit. Genelute hp endotoxinfree plasmid dna maxiprep kit 25. To reorder product call 18003253010, visit our web site at. A comparatively inexpensive one is from sigma genelute endotoxinfree plasmid midiprep kit.
Name genelute plasmid miniprep kit product number pln10 brand sigma company sigmaaldrich address 3050 spruce street saint louis mo 63103 us. The geneaid maxiprep plasmid kit was designed for plasmid dna purification using prepacked anionexchange resin maxiprep plasmid columns to purify plasmid dna from 2001600 ml of cultured bacterial cells. Qiagen plasmid plus midi kit en print bookmark share pdf 57kb english format file size language download get adobe reader contact qiagen. Millipore sigma purification kits crossreference tool. Coppermediated dna damage by the neurotransmitter dopamine and ldopa. Designed for rapid small scale isolation and purification of high copy and low copy plasmid dna. Genelute hp high performance plasmid midiprep kit na0200 bulletin free download as pdf file. Description the kit utilizes silicabased membrane technology in the form of convenient spin column. Zymopure ii plasmid maxiprep kit upgraded from zymopure.
This technology is based on alkaline lysis and puri. What is your preferred maxi prep kit for plasmid dna grown. Millipore sigma purification kits crossreference tool from qiagen. If you will be using a kit, follow the kits instructions. Genelute hp plasmid dna maxiprep kit 25 purifications sigma. Genelute hp endotoxinfree plasmid maxiprep kit vs zymopure plasmid maxiprep kit this page will help you compare kit specs and protocols across manufacturers. Cloning, sequencing, pcr, transformation and restriction analysis. The genelute hp endotoxinfree plasmid maxiprep kit offers a simple and rapid method for isolating endotoxinfree plasmid dna from recombinant e.
As research moves from dna sequencing to analysis of gene function, the need has increased for rapid methods for isolating large quantities of highquality plasmid dna. Kit 250 preps na0200s genelute hp plasmid midiprep kit prepsna0200 genelute hp plasmid midiprep kit 25 preps na0300s genelute hp plasmid maxiprep kit. Maxi kit 12863 sigma genelute hp plasmid maxiprep kit na0300, na0310, genelute hp. There are a number of similar products on the market but this has been used in most laboratories i have worked in, its reputation clearly speaks for itself. The plasmids were quantified by spectrophotometry and size was confirmed by agarose gel electrophoresis. The absence of cpg in plasmid dna chitosan polyplexes. Na0300 genelute hp plasmid maxiprep kit 10 preps faster 30 min.
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